OPTIMAL KINETIC PARAMETERS AND BATCH MODELING FOR PECTIN HYDROLYSIS TO GALACTURONIC ACID WITH PECTINEX ULTRA SP-L ENZYME |
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Authors: | Ali Asghar Ghorbanpour Khamseh Michele Miccio Giovanna Ferrari |
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Affiliation: | 1. Department of Industrial Engineering (formerly Department of Chemical and Food Engineering) , University of Salerno , Fisciano , Italy aghorbanpourkhamse@unisa.it aligh1966@gmail.com;3. Department of Industrial Engineering (formerly Department of Chemical and Food Engineering) , University of Salerno , Fisciano , Italy;4. Department of Industrial Engineering (formerly Department of Chemical and Food Engineering) , University of Salerno , Fisciano , Italy;5. Prodal S.c.ar.l. , Fisciano , Italy |
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Abstract: | Galacturonic acid is a monosaccharide obtained by pectin hydrolysis and a suitable substrate to produce bioethanol by fermentation. This article focuses on quantification of citrus pectin hydrolysis to galacturonic acid and provides new, reliable kinetic parameters for the Michaelis-Menten equation when the well-known commercial Pectinex Ultra SP-L is employed as enzyme. They are: r max = 1.10 g/(L min), K m = 10.42 g/L, and K IGA = 10.05 g/L, as obtained with a great accuracy by a nonlinear regression method and confirmed by the three classical linearization procedures (Lineweaver-Burk, Langmuir, and Eadie-Hofstee). The quantification of product inhibition has been achieved, with its inclusion in the rate equation. A batch reactor model yields perfect agreement between predictions and experiments, even under conditions different from those on which the parameters had been determined by regression. |
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Keywords: | Citrus pectin Enzymatic hydrolysis Galacturonic acid Michaelis-menten equation Nonlinear regression Pectinex Ultra SP-L |
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