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OBTAINING SCARLET SAGE (SALVIA COCCINEA) EXTRACT THROUGH HOMOGENIZER- AND ULTRASOUND-ASSISTED EXTRACTION METHODS
Authors:Mehmet Bilgin  Selin Sahin  M Umur Dramur  Lutfullah M Sevgili
Affiliation:1. Department of Chemical Engineering , Engineering Faculty, Istanbul University , Avcilar , Istanbul , Turkey mbilgin@istanbul.edu.tr;3. Department of Chemical Engineering , Engineering Faculty, Istanbul University , Avcilar , Istanbul , Turkey
Abstract:Scarlet sage (Salvia coccinea) leaves were extracted with water, ethanol, methanol, and aqueous mixtures of those solvents by homogenizer-assisted extraction (HAE) and ultrasound-assisted extraction (UAE) methods, which are novel technologies used for this purpose. The best results were compared with those obtained by the conventional Soxhlet method (SM). Total phenolic content was expressed in gallic acid equivalent (GAE) per g of dried leaf. Free radical scavenging activity for the antioxidant capacity was tested by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical. The greatest amount of extraction was achieved by pure water with the value of 368.87 mg/g dried matter and the highest total phenolic content was observed by 100% methanol as 43.18 mg-GAE/g dried matter, both obtained by the HAE method. The mint leaves extracted by SM exhibited the highest antioxidant capacity, with a value of 90.55% for inhibition of DPPH radical.
Keywords:Antioxidant capacity  Homogenizer-assisted extraction  Phenolic compounds  Salvia coccinea  Soxhlet  Ultrasound-assisted extraction
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