OPTIMIZATION OF LIPASE-CATALYZED SYNTHESIS OF N-trans-FERULOYLTYRAMINE USING RESPONSE SURFACE METHODOLOGY (RSM) |
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| Authors: | Mahiran Basri Mohammad Abu Alrub Emilia Abd Malek Shahrul Ainliah Abu Bakar Salleh Mohd Basyaruddin Abdul Rahman |
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| Affiliation: | 1. Faculty of Biotechnology and Biomolecular Science, Faculty of Science , University Putra Malaysia , Serdang , Selangor , Malaysia mahiran@science.upm.edu.my;3. Faculty of Biotechnology and Biomolecular Science, Faculty of Science , University Putra Malaysia , Serdang , Selangor , Malaysia |
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| Abstract: | Enzymatic synthesis of N-trans-feruloyltyramine amide was optimized by response surface methodology (RSM) using 4-hydroxy-3-methoxycinnamic acid and tyramine hydrochloride in a one-step lipase catalyzed reaction using Lipozyme TL IM. Response surface methodology (RSM) based on five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interaction of synthesis, reaction time (24–96 h), temperature (30°–50°C), amount of enzyme (50–500 mg, 12.5–125.0 IUN), and substrate molar ratio (cinnamic acid:tyramine HCl) 1:1–8:1 mmol on the percentage yield of N-trans-feruloyltyramine amide. The optimum conditions derived via RSM were: reaction time 52 h, temperature 43°C, amount of enzyme 260 mg (65.0 IUN), and substrate molar ratio (cinnamic acid:tyramine HCl) 6.2:1. The actual experimental yield was 96.3% under optimum conditions, which compared well to the maximum predicted value of 97.2%. |
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| Keywords: | Cinnamic acid Enzymatic synthesis Lipozyme N-trans-feruloyltyramine RSM Tyramine HCL |
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