| 干酪乳杆菌GL-B体外降胆固醇的作用机理 |
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| 引用本文： | 纪旭,侯红漫,王秀玲,孙思. 干酪乳杆菌GL-B体外降胆固醇的作用机理[J]. 中国乳品工业, 2010, 38(4) |
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| 作者姓名： | 纪旭 侯红漫 王秀玲 孙思 |
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| 作者单位： | 大连工业大学生物与食品工程学院,辽宁,大连,116034 |
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| 基金项目： | 辽宁省教育厅创新团队项目 |
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| 摘 要： | 研究了干酪乳杆菌GL-B所产胆盐水解酶的分离纯化及其体外降解胆固醇的作用机理,通过硫酸铵沉淀、DEAE-Sepharose FastFlow层析、Sephadex G-100层析分离胆盐水解酶。结果表明,胆盐水解酶纯度为原来的27倍,纯化后电泳谱图显示一条带,分子量约为50 ku;干酪乳杆菌GL-B对胆固醇的降解是吸收和共沉淀共同作用的结果,且共沉淀作用大于吸收作用。
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| 关 键 词： | 胆固醇降解 胆盐水解酶 分离纯化 |
Mechanism on L.casei ZP-W cholesterol-lowering in vitro |
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| JI Xu,HOU Hong-man,WANG Xiu-ling,SUN Si. Mechanism on L.casei ZP-W cholesterol-lowering in vitro[J]. China Dairy Industry, 2010, 38(4) |
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| Authors: | JI Xu HOU Hong-man WANG Xiu-ling SUN Si |
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| Affiliation: | JI Xu,HOU Hong-man,WANG Xiu-ling,SUN Si(College of Bio & Food Technology,Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | Bile salt hydrolase produced by L.casei GL-B,Used to study the method of purification and mechanism in the cholesterol-lowering ex-periment in vitro.The bile salt hydrolase was purified by ammonium sulphate precipitation,followed by DEAE-Sepharose Fast Flow exchange chromatography and sephadex G-100.Resrults showed that purity quotient of Bile salt hydrolase becamed 27 times of primary commodity,its molecular weights in SDS-PAGE electrophoresis was 50 ku.L.casei GL-B induced the lowering of cholesterol by a... |
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| Keywords: | cholestorol-lowering bile salt hydrolase purification |
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