A new family of sugar-inducible expression vectors for Escherichia coli |
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Authors: | Cagnon, Christine Valverde, Viviane Masson, Jean-Michel |
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Affiliation: | INSA, Laboratoire de Génie Biochimique et Alimentaire, URA 544 du CNRS, Centre de transfert en Biotechnologie-Microbiologie, Complexe Scientifique de Rangueil 31077 Toulouse Cedex, France |
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Abstract: | A set of 11 expression vectors was constructed, each of themharbouring a cloning cassette under the control of the promoter.Some of these vectors enable expression of foreign proteinsin the cytoplasm, while others include a synthetic sequencecoding for a very efficient secretion signal sequence. Otherfeatures are an fl origin of replication (in plus or minus orientation)and a promoterup mutation that enhances the already very highlevel of expression from these vectors. With such a versatilevector family, cloning, sequencing and sitedirected mutagenesiscan be performed on the same vector, and the level of expressioncan be defined according to the specific constraints of a givenprotein. |
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Keywords: | arabinose promotor/ bleomycin binding protein/ ß -galactosidase assay/ secretion vectors |
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