Deposition of Lead Phosphate by Lead-Tolerant Bacteria Isolated from Fresh Water near an Abandoned Mine |
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| Authors: | Yugo Kato Satoshi Kimura Toshihiro Kogure Michio Suzuki |
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| Affiliation: | 1.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan; (Y.K.); (S.K.);2.Department of Earth and Planetary Science, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan;3.Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo 113-8657, Japan |
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| Abstract: | Specialist bacteria can synthesize nanoparticles from various metal ions in solution. Metal recovery with high efficiency can be achieved by metal-tolerant microorganisms that proliferate in a concentrated metal solution. In this study, we isolated bacteria (Pseudomonas sp. strain KKY-29) from a bacterial library collected from water near an abandoned mine in Komatsu City, Ishikawa Prefecture, Japan. KKY-29 was maintained in nutrient medium with lead acetate and synthesized hydrocerussite and pyromorphite nanoparticles inside the cell; KKY-29 also survived nanoparticle synthesis. Quantitative PCR analysis of genes related to phosphate metabolism showed that KKY-29 decomposed organic phosphorus to synthesize lead phosphate. KKY-29 also deposited various metal ions and synthesized metal nanoparticles when incubated in various metal salt solutions other than lead. The present study considers the development of biotechnology to recover lead as an economically valuable material. |
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| Keywords: | bacteria bioremediation lead metal nanoparticles screening |
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