| 绿茶多糖的扫描电镜制样新方法及原子力显微镜观察 |
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| 引用本文： | 秦利鸿,曹建波,易伟松.绿茶多糖的扫描电镜制样新方法及原子力显微镜观察[J].电子显微学报,2009,28(2):162-167. |
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| 作者姓名： | 秦利鸿 曹建波 易伟松 |
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| 作者单位： | 1. 华中农业大学,电镜平台,湖北,武汉,430070
2. 华中农业大学,理学院,湖北,武汉,430070 |
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| 基金项目： | 华中农业大学科技创新项目，武汉市科技攻关项目 |
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| 摘 要： | 利用扫描电镜（SEM）和原子力显微镜（AFM）对绿茶多糖及酶解绿茶多糖试样进行显微成像,得到颗粒状或由单个颗粒聚集成的形貌结构。结果表明：改进制样方法的扫描电镜观察结果与原子力显微镜成像基本一致,说明了改进后的扫描电镜制样方法是一种简单有效的方法。实验结果还在一定程度上解释了绿茶多糖经酶解后其微观形态的变化情况。
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| 关 键 词： | 绿茶多糖 扫描电镜 原子力显微镜 |
A new method for scanning electron microscope samples of polysaccharides distilled from green tea and the observation of ultrafine structure of them with atomic force microscope |
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| QIN Li-hong,CAO Jian-bo,YI Wei-song.A new method for scanning electron microscope samples of polysaccharides distilled from green tea and the observation of ultrafine structure of them with atomic force microscope[J].Journal of Chinese Electron Microscopy Society,2009,28(2):162-167. |
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| Authors: | QIN Li-hong CAO Jian-bo YI Wei-song |
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| Affiliation: | 1. The Public Labratory of Electron Microscope;Huazhong Agricultural University;Wuhan Hubei 430070;2. College of Science Huazhong Agricultural University;China |
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| Abstract: | A new way has been found that dropping the polysaccharides on a fresh mica,fixing them with 1% OsO4 for two hours,sticking them on sample holder directly and plating them with platinum,then a clear figure of microdot structure was under the scanning electron microscope. The fine microstructure of polysaccharides distilled from green tea which was in accordance with the results from scanning electron microscope was more studied with the tipping mode atomic force microscope and show how the advanced structure... |
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| Keywords: | polysaccharides distilled from green tea scanning electron microscope atomic force microscope |
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