高效液相色谱法测定草酸艾司西酞普兰中的有关物质

建立了草酸艾司西酞普兰中有关物质测定的高效液相色谱测定方法。采用高效液相色谱法,用十八烷基硅烷键合硅胶为填充剂;以0.05 mol/L磷酸二氢钾（0.5%三乙胺,用磷酸调节pH至4.0）—甲醇（45∶55）为流动相,流速为1.0 mL/min;检测波长为240 nm;柱温：25℃。草酸艾司西酞普兰与各杂质分离良好,最低检测限为5 ng。该方法简单、快速、准确、灵敏度高、专属性好,可用于草酸艾司西酞普兰原料有关物质的检查。

Determination of Related Substances of Escitalopram Oxalate with High Performance Liquid Chromatography

The method for the determination of related substance of escitalopram oxalate with high performance liquid chromalography was established.The chromatographic column was C18(150 mm×4.6 mm,5 μm).The mobile phase consisted of 0.05 mol/L potassium dihydrogen phosphate solution(0.5% trithylamine,adjusted pH to 4.0 by phosphoric acid)-methanol(45:55).The flow rate was 1.0 mL/min,the detection wavelength was 240 nm,and the column temperature was 25 ℃.Escitalopram oxalate was separated from the impurities thoroughly.The LOD was 5 ng for escitalopram oxalate.The method was simple,specific,sensitive and suitable for the determination of related substances of escitalopram oxalate.