Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations |
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Authors: | Hassane Oudadesse Eric Wers Xuang Vuong Bui Claire Roiland Bruno Bureau Imane Akhiyat Amany Mostafa Hassan Chaair Hicham Benhayoune Joel Fauré Pascal Pellen-Mussi |
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Affiliation: | 1. SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France 2. Faculté des Sciences et Techniques de Mohammedia (FSTM), Université Hassan II, Maroc, France 3. Biomaterilas Department, National Research Centre (NRC), 12622, Dokki, Cairo, Egypt 4. LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France
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Abstract: | Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, Q Si 3 (OH) and Q Si 4 , which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment. |
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