Sigmoidal thermal inactivation kinetics of Listeria innocua in broth: Influence of strain and growth phase |
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Authors: | F tima A. Miller, Maria M. Gil, Teresa R.S. Brand o, Paula Teixeira,Cristina L.M. Silva |
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Affiliation: | aUniversidade Católica Portuguesa, Escola Superior de Biotecnologia, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal |
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Abstract: | Listeria innocua inactivation was studied within the temperature range 52.5–65.0 °C, comparing two different strains (10528 and 2030c) and two growth phases (exponential and stationary). Survival curves may present a sigmoidal behaviour, with an initial shoulder (L), followed by a maximum inactivation rate (kmax) period and a final tailing tendency. A Gompertz-inspired model was used to fit experimental data, and kinetic parameters (L, kmax and tail) were estimated by non-linear regression analysis. The influence of temperature, growth phase and strain on kinetic parameters was studied using a 23 factorial experimental design. Results showed that temperature and growth phase were the most significant variables affecting the kinetic parameters. Listeria thermal inactivation varied from a log-linear tendency till a pronounced sigmoidal behaviour, depending on the studied factors. |
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Keywords: | Listeria innocua Thermal inactivation Growth phase |
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