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| 烟草抗番茄斑萎病毒基因筛选与生物信息学分析 | |
| 引用本文： | 杨金广,樊韦华,杨柳青,丁涛,王耀峰,宋玉川,董昕,王凤龙,刘晓璐. 烟草抗番茄斑萎病毒基因筛选与生物信息学分析[J]. 中国烟草科学, 2016, 37(4): 60-67. DOI: 10.13496/j.issn.1007-5119.2016.04.011 |
| 作者姓名： | 杨金广 樊韦华 杨柳青 丁涛 王耀峰 宋玉川 董昕 王凤龙 刘晓璐 |
| 作者单位： | 1. 中国农业科学院烟草研究所, 烟草行业烟草病虫害监测与综合治理重点实验室, 青岛 266101; 2. 北京科技大学化学与生物工程学院, 北京 100083; 3. 甘肃省烟草公司庆阳市公司, 甘肃 西峰 745000; 4. 云南烟草保山香料烟有限责任公司, 云南 保山 678000 |
| 基金项目： | 国家烟草专卖局科技创新平台项目“烟草行业烟草病虫害监测与综合治理重点实验室专项经费”(023201305);云南省烟草公司科技项目“德宏绿色生态优质烟叶生产技术研究及应用”(2013YN37) |
| 摘 要： | 通过同源克隆的方法在TSWV高感烟草品种NC89、TSWV耐病烟草品种中烟100中分别克隆出一个疑似抗番茄斑萎病毒(Tomato spotted wilt virus, TSWV)的基因，分别命名为NtSw-5NC89和NtSw-5zy100。序列分析表明，NtSw-5NC89长3819bp，包含1个完整的读码框，编码1273个氨基酸，理论等电点和相对分子质量分别为5.64和147529.5Da；NtSw-5zy100长3165bp，包含1个完整的读码框，编码1054个氨基酸，理论等电点和相对分子质量分别为6.75和122032.9Da；NtSw-5NC89和NtSw-5zy100均不含信号肽，均无明显跨膜区，二者均含有大多数植物抗性蛋白所共有的CC-(NB-ARC)-LRR典型结构。系统进化树分析表明，NtSw-5NC89与马铃薯假定晚疫病抗性蛋白同源R1A-3近源关系最近，NtSw-5zy100与烟草假定晚疫病抗性蛋白同源R1A-3同型X2近源关系最近。该结果可为烟草番茄斑萎病毒抗性研究和烟草番茄斑萎病毒的防治提供理论基础。 |
| 关 键 词： | 烟草 番茄斑萎病毒 抗性基因 生物信息学分析 |
| 收稿时间： | 2015-11-12 |
Screening and the Bioinformatics Analysis of the Nicotiana tabacum L. TSWV-Resistant Genes |
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| YANG Jinguang;FAN Weihua;YANG Liuqing;DING Tao;WANG Yaofeng;SONG Yuchuan;DONG Xin;WANG Fenglong;LIU Xiaolu. Screening and the Bioinformatics Analysis of the Nicotiana tabacum L. TSWV-Resistant Genes[J]. Chinese Tobacco Science, 2016, 37(4): 60-67. DOI: 10.13496/j.issn.1007-5119.2016.04.011 | |
| Authors: | YANG Jinguang FAN Weihua YANG Liuqing DING Tao WANG Yaofeng SONG Yuchuan DONG Xin WANG Fenglong LIU Xiaolu |
| Affiliation: | 1. The Tobacco Industry, Tobacco Plant Diseases and Pests Monitoring and Comprehensive Control Key Laboratory, Tobacco Research Institute of CAAS, Qingdao 266101, China;2. School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China;3. Gansu Tobacco Corporation Qingyang Tobacco Corporation, Xifeng 745000, Gansu, China;4. Yunnan Tobacco Baoshan Oriental Tobacco Co., Ltd., Baoshan 678000, Yunnan, China |
| Abstract: | Two putative TSWV-resistant genes, named NtSw-5NC89and NtSw-5zy100 respectively, were isolated from the TSWV-high sensitive Nicotiana tabacum L. NC89 and TSWV- resistant Nicotiana tabacumL.zhongyan 100 using homology-based cloning,. Sequence analysis showed that NtSw-5NC89 is 3819 bp long and contains an open reading frame which encodes a polypeptide of 1273 amino acids with the theoretical isoelectric point 5.64 and molecular mass 147529.5 Da. NtSw-5 zy100 is 3165 bp long and contains an open reading frame which encodes a polypeptide of 1054 amino acids with the theoretical isoelectric point6.75 and molecular mass 122032.9 Da. No signal peptide or transmembrane region was detected in NtSw-5NC89 or NtSw-5zy100 Both proteins contain a CC-(NB-ARC)-LRR typical structure which is shared by most plant resistance proteins. Phylogenetic tree analysis showed that NtSw-5NC89 had the highest similarity to the Solanum tuberosum putative late blight resistance protein homolog R1A-3 and NtSw-5zy100 had the highest similarity to the Nicotiana sylvestrisputative late blight resistance protein homolog R1A-3 isoform X2. Results of this study provide theoretical basis for resistance study, prevention and treatment of Nicotianatabacum L. TSWV. |
| Keywords: | Nicotiana tabacum L. TSWV resistance gene bioinformatics analysis |
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