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高效液相色谱法测定马铃薯块茎的绿原酸含量
引用本文:张建华,金黎平,谢开云,屈冬玉. 高效液相色谱法测定马铃薯块茎的绿原酸含量[J]. 食品科学, 2007, 28(5): 301-304
作者姓名:张建华  金黎平  谢开云  屈冬玉
作者单位:中国农业科学院蔬菜花卉研究所; 中国农业科学院蔬菜花卉研究所 北京100081; 北京100081;
基金项目:国家高技术研究发展计划(863计划)
摘    要:目的:建立测定马铃薯块茎中绿原酸含量的高效液相色谱方法,并测定不同品质特点的马铃薯的绿原酸含量。方法:对甲醇浓度、固液比和超声提取时间三个试验因素进行正交试验设计:ZOBAX C18色谱柱,250mm×4.6mm 5μm;流动相:水-甲醇-冰醋酸(20∶80∶2;30∶70∶2;40∶60∶2);甲醇-0.4%磷酸溶液(10∶90):流速: 1.0ml/min;柱温:室温;进样体积:5μl;检测波长为327nm。结果:精密度:RSD为3.2%,n=6,加样回收率为97.5%,提取条件为:甲醇浓度70%,固液比1∶100,超声提取时间40min。确定水∶甲醇∶冰醋酸=30∶70∶2为流动相。

关 键 词:高效液相色谱法   马铃薯   块茎   绿原酸  
文章编号:1002-6630(2007)05-0301-04
修稿时间:2006-06-26

Determination of Chlorogenic Acid of Potato Tuber by HPLC
ZHANG Jian-hua,JIN Li-ping,XIE Kai-yun,QU Dong-yu. Determination of Chlorogenic Acid of Potato Tuber by HPLC[J]. Food Science, 2007, 28(5): 301-304
Authors:ZHANG Jian-hua  JIN Li-ping  XIE Kai-yun  QU Dong-yu
Affiliation:Institute of Vegetable and Flower,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Abstract:Object:To establish a new HPLC method for determination of chlorogenic acid(CGA)in potato,and determine its content of CGA in five cultivars.Method:Orthogonal experiment for three factors:CH3OH concentration,ratio of solid and liquid and time of ultrasounic extraction.CGA was analyzed with C18column(250 mm×4.6 mm,5μm)and with detector at 327 nm.The mobile phase consisting HO-CH3OH-CH3COOH(20:80:2;30:70:2;40:60:2 respectively),CH3OH-0.4% H3PO4 (10:90).Results:The recovery of CGA is 97.5%,precision(RSD)3.2%(n=6);CGA extraction conditions are:70% CH3OH,ratio of solid and liquid=1:100,and ultrasounic extraction time 40 min with determined mobile phase H2O-CH3OH-CH3COOH=30: 70:2.
Keywords:HPLC   potato   tuber   chlorogenic acid
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