LC-MS/MS法测定水基胶中的3种异噻唑啉酮类防腐剂

建立了测定水基胶中3 种异噻唑啉酮的液相色谱-串联质谱(LC-MS/MS)方法。水基胶样品经水萃取、离心后进行LCMS/MS 分析,内标法定量。结果表明:MI、CMI 在浓度范围2.5~250 ng/mL、BIT 在浓度范围5~500 ng/mL 内,线性关系良好(R2 ≥ 0.9996),3 个加标水平的加标回收率在96.3%~109.6% 之间,平均相对标准偏差(RSD)在1.5%~6.7% 之间,方法的检出限(LOD)和定量限(LOQ)分别为0.011~0.015 mg/kg 和0.035~0.051 mg/kg。该方法适用于水基胶中MI、CMI、BIT 的快速测定。 

Determination of 3 isothiazolinone preservatives in water-based adhesive by LC-MS/MS method

A method for determining 3 isothiazolinone preservatives in water-based adhesive by high performance liquid chromatographytandem mass spectrometry (LC-MS/MS) was developed. The sample was extracted with water, centrifugated, and then analyzed by LCMS/MS and quantified by internal standard method. Results showed that: the linear relationship was good with determination coefficients R2 ≥ 0.9996 in the MI and CMI concentration range of 2.5~250 ng/mL and in the BIT concentration range of 5~500 ng/mL. The recoveries of standard addition ranged from 96.3% to 109.6% with relative standard deviation (RSD) of 1.5%~6.7%. The limit of detection was between 0.011 and 0.015 mg/kg, and that of quantification was between 0.035 and 0.051 mg/kg. It was concluded that this method was suitable for the determination of 3 isothiazolinone preservatives in water-based adhesive.