Mitigating the variability of hydrogen production in mixed culture through bioaugmentation with exogenous pure strains |
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| Affiliation: | 1. School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, Republic of Korea;2. School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea;3. Department of Chemical Engineering, Kyonggi University, Suwon 16227, Republic of Korea;1. Department of Physics, Zhejiang University, Hangzhou, 310027, PR China;2. Zhejiang Province Key Laboratory of Quantum Technology and Device, Zhejiang University, Hangzhou, 310027, PR China;1. Key Laboratory of Energy Materials Chemistry, Ministry of Education, Institute of Applied Chemistry, Xinjiang University, Shengli Road No. 666, Urumqi, 830046, China;2. Key Laboratory of Advanced Functional Materials, Autonomous Region, Institute of Applied Chemistry, Xinjiang University, Shengli Road No. 666, Urumqi, 830046, China;3. Physics and Chemistry Detecting Center, Xinjiang University, Shengli Road No. 666, Urumqi, 830046, China;1. School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea;2. Intelligent Sustainable R&D Group, Korea Institute of Industrial Technology (KITECH), Chonan-si, Chungnam, South Korea;3. School of Civil and Environmental Engineering, Yonsei University, Seoul, South Korea;4. KU-KIST Green School, Graduate School of Energy and Environment, Korea University, Seoul, South Korea |
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| Abstract: | Process stability is a key operational issue when operating dark fermentation with mixed microbial cultures for hydrogen production. This study aimed at mitigating the instability of hydrogen production by separately adding exogenous pure strains suspected to have key roles in fermentative cultures. Among them, Clostridium acetobutylicum, Clostridium pasteurianum and Lactobacillus bulgaris which became predominant within the mixed culture strongly reduced the spectrum of produced metabolites and H2 production variability. Interestingly, Escherichia coli and Cupriavidus necator, which remained in minor abundance, maintained a high and stable H2 production while lowering the metabolic variability. 16S rRNA revealed that this could correlate to a simplification of the microbial diversity and the non-emergence of spore-forming competitors such as Sporolactobacillus sp. These results illustrate the potential beneficial role of minor OTUs as keystone species on H2-producing complex ecosystem and support the possibility of using them to engineer the ecosystem and maintain high and stable performances. |
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| Keywords: | Biohydrogen Bioaugmentation Dark fermentation Ecological engineering |
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