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Hydrogen-rich syngas fermentation for bioethanol production using Sacharomyces cerevisiea
Affiliation:1. Faculty of Engineering Technology, Universiti Malaysia Pahang, 26300 Gambang, Malaysia;2. Department of Petroleum and Mining Engineering, Jashore University of Science and Technology, Jashore, 7408, Bangladesh;3. Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh;4. Bioenvironmental Engineering Research Unit (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia;1. Department of Environmental Sciences, Faculty of Science, Damietta University, 34517 Damietta, Egypt;2. Department of Environmental Biotechnology, City of Scientific Research and Technology Applications, Alexandria, 21934, Egypt;3. Department of Environmental Engineering, Building 113, Technical University of Denmark, DK-2800 Lyngby, Denmark;1. Chemical Engineering Laboratory, Faculty of Sciences and Center for Advanced Scientific Research (CICA), University of La Coruña (UDC), E-15008 La Coruña, Spain;2. UNESCO-IHE Institute for Water Education, Westvest 7, P.O. Box 3015, NL-2611 AX Delft, The Netherlands;1. Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA;2. Department of Chemical Engineering, Brigham Young University, Provo, UT, USA;1. Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA;2. Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA;1. Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA;2. Center for Sustainable and Environmental Technologies, Iowa State University, Ames, IA 50011, USA;1. Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK, USA;2. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, USA
Abstract:Bioethanol is an eco-friendly biofuel due to its merit that makes it a top-tier fuel. The present study emphasized on bioethanol production from hydrogen-rich syngas through fermentation using Sacharomyces cerevisiea. Syngas fermentation was performed in a tar free fermenter using a syngas mixture of 13.05% H2, 22.92% CO, 7.9% CO2, and 1.13% CH4, by volume. In the fermentation process, effects of various parameters including syngas impurity, temperature, pH, colony forming unit, total organic carbon and syngas composition were investigated. The yield of bioethanol was identified by Gas chromatography-Mass spectrometry analysis and further, it was confirmed by Nuclear magnetic resonance (1H) analysis. From GC-MS results, it is revealed that the concentration of bioethanol using Saccharomyces cerevisiae was 30.56 mmol from 1 L of syngas. Thus, hydrogen-rich syngas is suited for bioethanol production through syngas fermentation using Saccharomyces cerevisiae. This research may contribute to affordable and environment-friendly bioethanol-based energy to decrease the dependency on fossil fuels.
Keywords:Hydrogen-rich syngas  Bioethanol  Syngas fermentation  TFF
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