醛类-DNA 加合物检测技术研究进展

甲醛、乙醛、丙烯醛和巴豆醛广泛存在于环境污染物中,卷烟烟气中也有微量存在,它们不需要经过机体代谢就能够直接进攻亲核基团,可与 DNA分子发生共价结合形成 DNA加合物。DNA加合物是 DNA损伤的一种形式,在 DNA复制过程中可使所携带的遗传信息发生改变,从而有可能造成机体的损伤。本文综述了生物体内常见的 6种醛类-DNA加合物的检测技术以及醛类-DNA加合物作为卷烟烟气暴露的生物标志物的研究进展,展望了同时检测多种 DNA加合物的研究方向及醛类-DNA加合物作为 DNA损伤标志物的研究前景。 

Research progress in detection technology of DNA adducts derived from aldehydes

Formaldehyde, acetaldehyde, acrolein and crotonaldehyde exist widely in environmental pollutants and cigarette smoke also contains trace amounts of these aldehyde compounds. They can cause damage by attacking nucleophilic groups in the body directly. A covalent bond can be formed with DNA molecule to form DNA adducts. The formation of DNA adducts is a form of damage of DNA, which can change genetic information in the process of DNA replication. This paper reviewed six typical DNA adducts derived from aldehydes in organism and their research methods and progress of using aldehydes-DNA adducts as biomarkers in cigarette smoke exposure. Simultaneous quantitative analysis of multiple DNA adducts for cigarette smoke and its future development were also discussed.