| 利用基因芯片筛选烤烟早花基因初探 |
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| 引用本文： | 杨静,陈杰,陈建军,邓世媛,邱妙文,赵伟才,王维. 利用基因芯片筛选烤烟早花基因初探[J]. 中国烟草学报, 2015, 21(3): 111-118. DOI: 10.16472/j.chinatobacco.2014.312 |
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| 作者姓名： | 杨静 陈杰 陈建军 邓世媛 邱妙文 赵伟才 王维 |
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| 作者单位： | 1 黔东南州烟草公司镇远县分公司, 贵州镇远 557700; |
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| 基金项目： | 国家烟草专卖局重点科技项目“耐低温抗早花烤烟新品种选育及遗传生理机理研究”(110200902043);广东省烟草专卖局(公司)科技项目“耐低温抗早花烤烟新品种选育”(200816) |
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| 摘 要： | 为研究与烟草早花有关的基因,本试验利用安捷伦烟草全基因组基因芯片对烤烟品种K326 及其抗早花变异系华烟06、K326LF 花芽分化过程茎尖端基因表达谱进行分析,筛选出其中与花芽分化可能有主要关系的基因:AGL20、CCR2、ELF4、ERD7、CBF1、COR413-PM1、EMF2、VIP4、COL1、AGL17、COL9、FPA、SWN 等,其中COL2、ELF4 的表达差异达到了5倍;AGL62、CBF1、SWN、NAP 与FPA 的差异大于或等于10 倍。该试验可为进一步研究烟草抗早花机理及培育抗早花新品种提供参考。
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| 关 键 词： | 烤烟 早花 抗早花 基因芯片 基因 |
| 收稿时间： | 2014-08-29 |
A preliminary research on screening of premature flowering gene ni flue-cured tobacco by microarray |
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| Affiliation: | 1 Qiandongnan Zhenyuan County Tobacco Company, Zhenyuan 557700, Guizhou, China;2 College of Agriculture, South China Agricultural University, Guangzhou 510642, China;3 Qiandongnan Tobacco Company, Kaili 556000, Guizhou, China;4 Nanxiong Institute of Tobacco Science, Nanxiong 5124002, Guangdong, China |
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| Abstract: | Agilent Tobacco Oligo Microarray was used to deduce expression profiling of flue-cured tobacco K326 and its premature flowering resistance variety Huayan06, K326LF during flower bud differentiation process. Genes related to flower bud differentiation were screened out: AGL20,CCR2,ELF4,ERD7,CBF1,COR413-PM1,EMF2,VIP4,COL1,AGL17,COL9,FPA and SWN, etc. Among them, COL2 and ELF4 had 5 times difference in terms of expression level while AGL62, CBF1, SWN, NAP had 10 times or above difference with FPA. The study provided a reference for further study of flue-cured tobacco premature flowering resistance mechanism and premature flowering resistance varieties breeding. |
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