造纸法再造烟叶工艺流程中5种多酚的变化

利用超高效液相-二极管阵列检测器(UPLC-PDA)分析研究了造纸法再造烟叶各工艺环节产物中5 种多酚(绿原酸、咖啡酸、莨菪亭、芸香苷及愈创木酚)的含量及其影响因素。结果表明:①再造烟叶原料中89.2% 的多酚存在于碎烟叶中,烟梗中只有10.8%,其中原料中3 种主要多酚(绿原酸,莨菪亭,芸香苷)占多酚总量的97.2%;②提取阶段,原料中90.3% 的多酚转移到提取液中,二级提取固相物中多酚为9.7%,由于二级提取液相物的循环使用,最终提取液中多酚为97.1%;③真空浓缩阶段,绿原酸受热分解,损失量相当于多酚总量的10.0%,其余多酚变化不明显,最终涂布液中多酚占总量的89.7%;④打浆和抄造阶段,多酚随着水的流失而损失,片基中多酚剩余0.9%;⑤涂布阶段,多酚在有氧及高温下发生氧化,损失多酚48.7%,最终再造烟叶中多酚剩余41.9%。 

Dynamic variation of five polyphenols contents in processes of paper-making reconstituted tobacco production

Five polyphenols (chlorogenic acid, caffeic acid, scopoletin, rutin and guaiacol) generated from various paper-making reconstituted tobacco processes were analyzed by ultra performance liquid-diode array detector (UPLC-PDA). Impact of processing techniques on contents of polyphenols was also studied. Results showed that: ① 89.2% of polyphenol compounds existed in crushed tobacco leaves and rest in stems. Three major polyphenols, namely chlorogenic acid, scopoletin and rutin, accounted for 97.2% of the total; ② In extraction phase, 90.3% of polyphenol compounds in raw material was transferred, and the rest 9.7% was in the secondary extraction solid phase and the final extraction had 97.1% of polyphenol due to the cycling use of secondary extract liquid phase. ③ In vacuum concentration phase, about 10.0% chlorogenic acid was lost due to thermal decomposition, and the rest showed no obvious change. Polyphenols in final coating liquid accounted for about 89.7% of the total; ④ In beating and paper-making phase, polyphenols decreased sharply with loss of water and only about 0.9% polyphenols remained in base-sheet owing to water soluble polyphenol compounds; ⑤ 48.7% of polyphenols was lost in coating phase due to oxidation and high temperature, and 41.9% of polyphenols remained in the final reconstituted tobacco.