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| 烟草内生细菌YN201448的定殖能力研究 | |
| 引用本文： | 杨珍福,何鹏飞,吴毅歆,毛自朝,何月秋.烟草内生细菌YN201448的定殖能力研究[J].中国烟草科学,2015,36(3):80-85. |
| 作者姓名： | 杨珍福 何鹏飞 吴毅歆 毛自朝 何月秋 |
| 作者单位： | 1. 云南农业大学植物保护学院, 昆明 650201; 2. 云南农业大学农学与生物技术学院, 昆明 650201; 3. 微生物菌株筛选与应用技术国家地方联合共建工程中心, 昆明 650471 |
| 基金项目： | 科技部国际科技合作项目“防治植物病害和促进植物生长的微生物开发应用及机制研究”(2009DFA326360);中国烟草总公司云南省公司项目“普洱市烤烟‘两黑病’综合防控技术研究与应用”(2015YN26) |
| 摘 要： | 为测定烟草内生细菌YN201448的定殖能力,通过自然转化法将携带绿色荧光蛋白基因的质粒pGFP4412成功导入YN201448细胞中,获得了GFP标记菌株48-pGFP。48-pGFP能稳定表达GFP蛋白,且对5种病原真菌的拮抗能力与野生型YN201448的相同。用48-pGFP菌液浸泡烟草种子和浇灌烟草苗后,在烟草的根、茎、叶等组织中都能检测到标记菌,其定殖数量分布为根 > 茎 > 叶;同时标记菌也能在根际土中稳定地定殖。激光共聚焦显微镜观察发现标记菌株主要聚集在烟草茎组织的表皮、皮层部位及维管组织。因此,YN201448可以在烟草体内外较长期定殖。 |
| 关 键 词： | 解淀粉芽孢杆菌 绿色荧光蛋白 拮抗作用 自然转化 |
| 收稿时间： | 2014-08-31 |
The Colonization of Endophytic Bacterium YN201448 in Tobacco |
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| YANG Zhenfu,HE Pengfei,WU Yixin,MAO Zichao,HE Yueqiu.The Colonization of Endophytic Bacterium YN201448 in Tobacco[J].Chinese Tobacco Science,2015,36(3):80-85. | |
| Authors: | YANG Zhenfu HE Pengfei WU Yixin MAO Zichao HE Yueqiu |
| Affiliation: | 1. Faculty of Plant Protection, Yunnan Agricultural University (YAU), Kunming 650201, China; 2. Faculty of Agronomy and Biotechnology, YAU, Kunming 650201, China; 3. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650471, China |
| Abstract: | In order to determine the colonization of endophytic bacterium YN201448 in tobacco, we successfully obtained a stable GFP tagged strain 48-pGFP by transferring the pGFP4412 plasmid into YN201448 via natural transformation. 48-pGFP showed the same antagonistic effect against 5 pathogenic fungi as the wild strain YN201448. 48-pGFP was observed to be distributed in root, stem and leaf tissues of tobacco after its seeds soaked and seedling roots drenched with 48-pGFP. The colonizing bacterium decreased with the trend of root > stem > leaf, and 48-pGFP could colonize in rhizospheric soil stably. Fluorescence microscope observation indicated that 48-pGFP mainly appeared in tobacco stem epidermis, cortex and vascular tissue cells. Therefore, YN201448 could colonize inside and around tobacco plant stably for a relatively long period of time. |
| Keywords: | Bacillus amyloliquefaciens GFP antagonistic effect natural transformation |
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