地黄试管苗组织培养基中矿质元素含量测定

目的建立测定地黄试管苗组织培养基中N,P,K元素含量的方法。方法稀释不同倍数MS培养液模拟不同培养时间的地黄试管苗组织培养基,分别用水杨酸硝化法、钼蓝法、离子选择电极法测定N,P,K元素的含量。结果实验结果表明,作者选用的测定方法简便、快速、易于操作。结果的精密度和准确度均能满足测定要求。结论建立的方法可实际应用于地黄组织培养基中N,P,K元素含量的测定。

Assay method of mineral elements in tissue culture medium of test-tube plantlet of Rehmannia glutinosa

Objective To establish a feasible assay methods of N,P and K element in tissue culture medium of test-tube plantlet of Rehmannia glutinosa.Method Contents of N,P and K in tissue culture medium of test-tube plantlet of rehmannia glutinosa were determined by the methods of salicylic acid nitrofication process,molybdenum blue method and ion selective electrode method,respectively.The culture medium had been diluted in different multiple.Result The methods were convenient,quick and manipulable.The accuracy and precision of the methods was suitable for the requirement of determination.Conclusion These methods could be used in practical production.