| 微生物降解纤维素机制的分子生物学研究进展 |
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| 引用本文： | 高培基,曲音波.微生物降解纤维素机制的分子生物学研究进展[J].纤维素科学与技术,1995,3(2):1-19. |
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| 作者姓名： | 高培基 曲音波 |
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| 作者单位： | 山东大学微生物研究所!济南，250100，山东大学微生物研究所!济南，250100，山东大学微生物研究所!济南，250100，山东大学微生物研究所!济南，250100 |
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| 基金项目： | 国家自然科学基金，中科院广州化学研究所纤维素化学开放研究实验室资助 |
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| 摘 要： | 对纤维素酶的分子生物学，主要是该酶对天然纤维性底物的降解机制研究进展作了简要评述，包括纤维素酶的一、二、三级结构、酶分子的多形性、纤维素酶族、酶基因克隆方法及表达和分泌中存在的问题、新酶分子的构建等。并介绍对细胞融合、单克隆抗体、DNA体外定位诱变、活性中心测定和糖基化方法等在纤维素酶降解研究中的应用。
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| 关 键 词： | 纤维素酶 纤维素生物降解 纤维素酶基因克隆 |
Advances of molecular biology in microbial degradation of cellulose |
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| Gao Peiji, Qu Yinbo, Wang Tianhong, Yan Baixu.Advances of molecular biology in microbial degradation of cellulose[J].Journal of Cellulose Science and Technology,1995,3(2):1-19. |
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| Authors: | Gao Peiji Qu Yinbo Wang Tianhong Yan Baixu |
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| Abstract: | The recent advances of research on celluinse in molecular biology, especialy degrnding mechanism to native celiulosic materials were summarised. The fuction of various domains of ceilulase coding sequencen, the occurrences of multiple forms of cellulase, the enzyme families analyzed to date and the gene cloning of enzme moleculars were discribed. The application of several techniques to cellulase study, such as cell fusion, mono-clone antibody, site-direted mutagenesis of target sequences and its protein engineering etc., were also outlined in this review. |
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| Keywords: | cellulase cellulose biodegradation gene cloning of cellulase |
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