| 蚕沙叶绿素铜配合物与DNA的相互作用 |
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| 引用本文： | 王文雄,司利平,刘海洋. 蚕沙叶绿素铜配合物与DNA的相互作用[J]. 广东化工, 2010, 37(9): 19-20,28 |
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| 作者姓名： | 王文雄 司利平 刘海洋 |
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| 作者单位： | [1]珠海市蓝清环保科技有限公司,广东珠海519015 [2]华南理工大学化学与化工学院,广东广州510640 |
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| 摘 要： | 文章从蚕沙中提取出叶绿素(CHL),并由此制备得到了蚕沙叶绿素铜配合物(Cu-CHL)。采用琼脂糖凝胶电泳实验研究了在白炽灯光照下Cu-CHL对pBR 322 DNA的切割能力。实验结果表明铜叶绿素对pBR 322 DNA具有光断裂能力,最佳断裂浓度范围为178.5～357μM。并采用紫外光谱、CD光谱和粘度法探讨了二者的结合模式,结果显示其为外部结合模式,结合常数为2.04×106m-1。
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| 关 键 词： | 蚕多 叶绿素 DNA 必断裂 |
The Interaction of Cu-chlorophyll Prepared from Silkworm Faeces with DNA |
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| Wang Wenxiong,Si Liping,Liu Haiyang. The Interaction of Cu-chlorophyll Prepared from Silkworm Faeces with DNA[J]. Guangdong Chemical Industry, 2010, 37(9): 19-20,28 |
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| Authors: | Wang Wenxiong Si Liping Liu Haiyang |
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| Affiliation: | 1. Zhuhai Lanqing Environmental Protection Technology Co., Ltd., Zhuhai 519015; 2. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China) |
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| Abstract: | Copper chlorophyll（Cu-CHL） complex was prepared using Chlorophyll extracted from silkworm faeces. Agarose gel electrophoresis was carried out to investigate the photoc cleavage ability of Cu-CHL to pBR 322 DNA. The results suggested that Cu-CHL could induce DNA cleavage upon irradiation, and the optimization cleavage concentration range was 178.5-357 μM. Relative viscosity experiment, UV-Visible were used to study the interaction between Cu-CHL and ct-DNA. The results suggested that the binding mode of Cu-CHL and ct-DNA was perhaps the partly intercalative mode, the binding constant was 1.89x 106 M^-1. |
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| Keywords: | silkworm faeces chlorophyll DNA photo cleavage |
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