戊型肝炎病毒在长春地区动物群中的流行病学研究

目的分析戊型肝炎病毒(HEV)在长春地区动物群中的感染情况及戊型肝炎病毒系统进化关系。方法用抗-HEV抗体试剂盒检测长春地区猪、牛、羊、鹿、鸡和马血清中的抗体;对部分血清用RT-PCR检测HEVRNA,并对PCR阳性产物进行克隆测序及序列分析。结果493份猪血清中有427份为抗-HEV抗体阳性,阳性率为86.61%;有5份为HEVRNA阳性,5株克隆的核苷酸序列的同源性为91.2%～99.1%,该5株克隆的序列在ORF2区348bp与1～4型间核苷酸同源性分别为77.8%～82.3%、77.2%～78.1%、77.2%～99.1%和85.2%～95.2%;266份牛血清中有122份为抗-HEV抗体阳性,阳性率为45.86%;93份羊血清中有7份为抗-HEV抗体阳性,阳性率为7.53%;798份鹿血清中有348份为抗-HEV抗体阳性,阳性率为43.61%;369份鸡血清中有18份为抗-HEV抗体阳性,阳性率为4.88%;197份马血清中有31份为抗-HEV抗体阳性,阳性率为15.74%。结论HEV在多种动物群中均有流行,但在猪群中的流行率明显高于其他动物群。猪感染的HEV的基因序列与人群中散发性戊型肝炎病毒的基因4型同源性最高。进化关系表明,在长春地区猪与人HEV应划为一个分支。

Prevalence of Hepatitis E Virus in Animals in Changchun Area, China

Objective To analyze the prevalence of hepatitis E virus(HEV)in animals in Changchun Area,China,as well as the phylogenetics of the virus.Methods The sera of pig,cattle,sheep,deer,chicken and horse were tested for antibody against HEV by anti-HEV antibody kit,a part of which for HEV RNA by RT-PCR,and the positive ones were subject to gene cloning and sequencing.Results Of 493 pig,266 cattle,93 sheep,798 deer,369 chicken and 197 horse sera,427(86.61%),122(45.86%),7(7.53%),348(43.61%),18(4.88%)and 31(15.74%)were positive for anti-HEV antibody respectively,and 5 of the 493 pig sera were positive for HEV RNA.The homology of the 5 HEV RNA positive clones was 91.2%-99.1%,and those of 348 bp at ORF2 domain to HEV genotype 1,2,3 and 4 were 77.8%-82.3%,77.2%-78.1%,77.2%-99.1% and 85.2%-95.2% respectively.Conclusion The prevalence of HEV was observed in various animals in Changchun Area.The prevalent in pigs was much higher than those in other animals.The HEV gene sequence prevalent in pigs showed high homology to that of HEV-4 isolated from sporadic HE case in humans.The analysis of phylogenetics proved that the HEV in pigs and humans in Changchun Area belonged to the same branch.