| 红外光谱法测定虎舌红提取物中的总黄酮 |
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| 引用本文： | 凌育赵,刘经亮. 红外光谱法测定虎舌红提取物中的总黄酮[J]. 化学与生物工程, 2011, 28(12): 92-94 |
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| 作者姓名： | 凌育赵 刘经亮 |
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| 作者单位： | 1. 仲恺农业工程学院化学化工学院,广东广州,510225
2. 广东食品药品职业学院,广东广州,510520 |
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| 基金项目： | 仲恺农业工程学院校级科研基金资助项目(G3100032) |
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| 摘 要： | 采用索氏回流法及溶剂萃取法成功地提取了虎舌红中的总黄酮,并对其结构进行了鉴定,采用红外光谱法对提取物中总黄酮的含量进行了测定.结果表明,虎舌红提取物中总黄酮含量为19.47 mg·g-1,与紫外可见分光光度法测定结果基本一致；相对标准偏差(RSD)为2.09％(n=6),加标回收率为93.54％～104.35％,平均回...
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| 关 键 词： | 虎舌红 黄酮类化合物 红外光谱 |
Quantitative Determination of Total Flavonoids in the Extract of Ardisia Mamillata by Infrared Spectroscopy |
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| LING Yu-zhao , LIU Jing-liang. Quantitative Determination of Total Flavonoids in the Extract of Ardisia Mamillata by Infrared Spectroscopy[J]. Chemistry & Bioengineering, 2011, 28(12): 92-94 |
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| Authors: | LING Yu-zhao LIU Jing-liang |
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| Affiliation: | 1.Department of Chemistry and Chemical Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China; 2.Guangdong Food and Drug Vocational College,Guangzhou 510520,China) |
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| Abstract: | Total flavonoids was extracted from Ardisia mamillata successfully by using the method of Soxhlet reflux and solvent extraction,and its structure was identified.The content of total flavonoids in the extract of Ardisia mamillata was determined by IR.The content of total flavonoids in the extract was 19.47 mg·g-1 which was consistent with that obtained using UV-Vis spectrophotometry.The RSD(n=6)was 2.09%,recoveries was 93.54%～104.35%,and the average recovery was 98.72%. |
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| Keywords: | Ardisia mamillata flavonoids infrared spectroscopy(IR) |
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