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灵芝菌在茶培养基中驯化的实验研究
引用本文:邹礼根,吴元锋.灵芝菌在茶培养基中驯化的实验研究[J].食品研究与开发,2006,27(1):17-20.
作者姓名:邹礼根  吴元锋
作者单位:1. 杭州市农业科学研究院,农产品加工研究所,杭州,310024
2. 浙江科技学院生物与化学工程学院,杭州,310012
摘    要:研究了灵芝菌经过低档绿茶萘汁、茶粉多次驯化后,平板培养时菌丝增殖速率和摇瓶发酵时菌体生物量与胞外粗多糖(EPS)的变化情况。实验结果表明,驯化种在茶汁平板和普通平板上,菌丝平均增殖速率分别提高了30.7%、8.0%;驯化种在茶培养基中发酵时,最大菌体生物量和胞外粗多糖产量分别提高了119.87%、69.39%。显然,驯化种发生了部分遗传特性良性改变。

关 键 词:低档绿茶  灵芝菌  菌体生物量  胞外粗多糖(EPS)
收稿时间:2005-03-01
修稿时间:2005年3月1日

THE EXPERIMENTAL RESEARCH ON DOMESTICATION OF GANODERMA LUCIDUM IN THE TEA MEDIUM
ZOU Li-gen,WU Yuan-feng.THE EXPERIMENTAL RESEARCH ON DOMESTICATION OF GANODERMA LUCIDUM IN THE TEA MEDIUM[J].Food Research and Developent,2006,27(1):17-20.
Authors:ZOU Li-gen  WU Yuan-feng
Affiliation:1.Institute of Fainting Products Processing, Hangzhou Academy of Agricultural Sciences,Hangzhou 310024; 2.School of Biological and Chemical Engineering.Zhejiang University of Science and Technology,Hangzhou,310012
Abstract:Gonoderma lucidum was repeatedly domesticated by tea liquor and tea powder. Then,the growth rate of mycelia in culture plates and the yield of cell biomass and EPS in shake flask fermentation were researched. The results indicated that the mycelia growth rate of domesticated strains in common and tea culture plates were raised by 8.0 %, 30.7 % respectively. Furthermore, the yield of cell biomass and EPS of domesticated strain in shake flask fermentation were increased by 119.87 %, 69.39 % respectively.Obviously,the tea domestication resulted to the positive genetic variation of Ganoderma lucidum.
Keywords:low-grade green tea  Ganoderma lucidum  the biomass yield  exopolysaccharides
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