兔脂肪间充质干细胞胰岛素分泌功能的体外诱导

目的研究兔脂肪间充质干细胞体外诱导分化为具有胰岛素分泌功能的细胞的可能性。方法采用贴壁法分离兔脂肪间充质干细胞,培养、传代后,以胰高糖素样多肽(GLP-1)和尼克酰胺作为诱导剂进行诱导分化。对诱导后的细胞进行双硫腙染色及葡萄糖刺激胰岛素分泌试验,ELISA法检测胰岛素含量。结果细胞诱导后逐渐聚集成细胞团,双硫腙染色呈砖红色,诱导后第14天培养液中可检测到胰岛素,第21天浓度较14d增高,高糖组[(21.5±1.6)μIU/ml]比低糖组[(18.1±2.5)μIU/ml]略高,且差异有显著意义。结论兔脂肪间充质干细胞在体外一定诱导条件下,具有向胰岛素分泌细胞分化的潜能。

In Vitro Induction of Differentiation of Rabbit Adipose-derived Stromal Cells to Insulin-secreting Cells

Objective To explore the possibility of differentiation of rabbit adipose-derived stromal cells to insulin-secreting cells by in vitro induction.Methods Rabbit adipose-derived stromal cells (rADSCs) were isolated by adherent culture,then incubated,subcultured and induced with glucagons-like polypeptide-1 (GLP-1) and nicotinic amide.The rADSCs after induction were subjected to dithiozone (DTZ) staining and insulin secretion test stimulated by glucose.The insulin content was determined by ELISA.Results The rADSCs were aggregated to clumps after induction,and were positive in DTZ staining.On day 14 after induction,insulin was detected in rADSCs,however,the insulin contents in rADSCs stimulated with high and low glucose concentrations showed no significant difference.On day 21,the insulin content increased significantly as compared with that on day 14,and was significantly higher in rADSCs stimulated with high glucose concentration than with low glucose concentration.Conclusion The rADSCs may be potentially differentiated to insulin-secreting cells under a certain condition for induction in vitro.