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产共轭亚油酸乳酸菌的筛选及生产条件的研究
引用本文:刘晓华,曹郁生,陈燕.产共轭亚油酸乳酸菌的筛选及生产条件的研究[J].现代食品科技,2003,19(Z1):3-5.
作者姓名:刘晓华  曹郁生  陈燕
作者单位:南昌大学中德联合研究院,南昌大学食品科学教育部重点实验室,南昌,330047
基金项目:江西省教育厅科研基金(NO:2001387)"酶法生产共轭亚油酸的研究"的部分内容.
摘    要:从分离到的乳酸菌中筛选到1株共轭亚油酸高产菌株,乳杆菌L1(Lactobacillus sp.),在MRS培养基上,乳杆菌L1产生共轭亚油酸的最适亚油酸的浓度是0.1%(v/v),最适培养时间是42h,参与共轭亚油酸形成的酶是胞内酶,并且可能是由多种酶共同作用的结果.建立了共轭亚油酸的紫外光谱扫描和高效液相色谱检测方法.

关 键 词:乳酸菌  共轭亚油酸  发酵条件
文章编号:1007-2764(2003)增刊-0002-03

Screening of Lactic Acid Bacteria that Produce Conjugated Linoleic Acid and the Fermentation Conditions of CLA
Xiaohua Liu,Yusheng Cao,Yan Chen.Screening of Lactic Acid Bacteria that Produce Conjugated Linoleic Acid and the Fermentation Conditions of CLA[J].Modern Food Science & Technology,2003,19(Z1):3-5.
Authors:Xiaohua Liu  Yusheng Cao  Yan Chen
Abstract:A high conjugated linoleic acid (CLA) producer, Lactobacillus L1 (Lactobacillus sp.) was screened from 36 strains of lactic acid bacteria. The optimum conditions for CLA production in MRS medium were that concentration of linoleic acid was 0.1%(v/v), incubation time was 42hs. The enzyme that formed CLA was intracellular. It could be a multienzyme reaction for the formation of CLA from linoleic acid. We also set up a UV-scan and high performance liquid chromatography methods to determine CLA in this research.
Keywords:Conjugated linoleic acid (CLA)  lactic acid bacteria  process conditions  
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