| 甘蔗叶多酚物质的超声提取及生理活性研究 |
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| 引用本文： | 阎欲晓,吴国燕,杨龙,粟桂娇. 甘蔗叶多酚物质的超声提取及生理活性研究[J]. 食品研究与开发, 2012, 33(4): 63-66 |
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| 作者姓名： | 阎欲晓 吴国燕 杨龙 粟桂娇 |
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| 作者单位： | 广西大学生命科学与技术学院,广西南宁,530004 |
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| 基金项目： | 广西大学科研基金资助项目(XGL090036) |
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| 摘 要： | 研究甘蔗叶多酚类物质的提取工艺及其生理活性。采用超声波辅助乙醇提取方法,通过单因素和正交试验对甘蔗叶中多酚类物质的提取工艺进行优化,并利用Fenton反应和邻苯三酚自氧化法检测多酚类物质对羟基自由基和超氧自由基的清除作用。结果表明,甘蔗叶多酚最佳提取工艺为固液比为1∶30(g/mL),超声功率为104 W,乙醇浓度为45%,超声时间为45 min,水浴温度为60℃。甘蔗叶多酚化合物提取液对羟自由基(OH.)、超氧阴离子自由基(O2-.)均有较好的清除作用。
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| 关 键 词： | 甘蔗叶 多酚 超声波 生理活性 清除能力 |
Ultrasonic-assisted Extraction and Physiological Activities of Polyphenols from Sugar Cane Leaves |
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| YAN Yu-xiao , WU Guo-yan , YANG Long , SU Gui-jiao. Ultrasonic-assisted Extraction and Physiological Activities of Polyphenols from Sugar Cane Leaves[J]. Food Research and Developent, 2012, 33(4): 63-66 |
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| Authors: | YAN Yu-xiao WU Guo-yan YANG Long SU Gui-jiao |
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| Affiliation: | (College of Life Science and Technology,Guangxi University,Nanning 530004,Guangxi,China) |
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| Abstract: | In this paper,it reports the use of one-factor-at-a-time and orthogonal array design methods to optimize the ultrasonic-assisted extraction of polyphenols from sugar cane leaves,followed by the evaluation of their scavenging activity of hydroxyl and superoxide radicals producted by fenton reaction and pyrogallol autoxidation.The results indicated that the optimum conditions for the extraction of polyphenols were determined to be: material-to-liquid ratio 1∶30(g/mL),ethanol concentration45 %,ultrasonic power 104 W and length of extraction time 45 min at 60 ℃.The crude extract product displayed a good scavenging activity to.OH and O2-.. |
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| Keywords: | sugar cane leaves polyphenols ultrasonic wave physiological avtivities scavenging activity |
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