| 利用玉米芯渣产纤维素酶条件的优化 |
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| 引用本文： | 赵光辉,王成福,李林,聂永来,李玉珍.利用玉米芯渣产纤维素酶条件的优化[J].食品研究与开发,2012(6):178-181. |
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| 作者姓名： | 赵光辉 王成福 李林 聂永来 李玉珍 |
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| 作者单位： | 山东福田药业有限公司 |
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| 摘 要： | 以玉米芯渣为培养基料,通过单因素及正交试验,对绿色木霉T9806菌株固态发酵产纤维素酶的培养条件进行摸索。结果表明,最佳培养基组成为,麸皮15.27%,玉米芯渣22.91%,(NH4)2SO40.4%,MgSO40.12%,CaCl20.12%,ZnSO40.08%,加水量61.1%。利用最佳培养基配方,在250 mL三角瓶中于35℃下恒温培养96 h,纤维素酶酶活可达到1.36×10-4kat/g(以干曲汁)。
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| 关 键 词： | 玉米芯渣 绿色木霉T9806 固态发酵 纤维素酶 |
Optimality Condition on Production of Cellulose Enzyme Using Corn Cob Residue |
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| ZHAO Guang-hui,WANG Cheng-fu,LI Lin,NIE Yong-lai,LI Yu-zhen.Optimality Condition on Production of Cellulose Enzyme Using Corn Cob Residue[J].Food Research and Developent,2012(6):178-181. |
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| Authors: | ZHAO Guang-hui WANG Cheng-fu LI Lin NIE Yong-lai LI Yu-zhen |
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| Affiliation: | (Shandong Futian Pharmaceutical Co.,Ltd.,Yucheng 251200,Shandong,China) |
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| Abstract: | Utilizing Corn cob residue as substrate the cellulose enzyme solid-state fermentation conditions for Trichoderma viride T9806 was studied through single factor experiments and orthogonal test.The results showed that optimal culture conditions were as follows: medium prepared from 22.91 % slag several,15.27 % wheat bran,0.4 %(NH4)2SO4,0.12 %MgSO4,0.12 %CaCl2,0.08 %ZnSO4 and 61.1 % water,at 35 ℃for 96 h.Under these optimal fermentation conditions,cellulose enzyme activity reached 1.36×10-4 kat/g dry medium. |
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| Keywords: | corn cob residue Trichoderma viride T9806 solid-state fermentation cellulose enzyme |
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