| 高效液相色谱法测定茶叶中五种水溶性维生素 |
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| 引用本文： | 郭玉华,郁有祝. 高效液相色谱法测定茶叶中五种水溶性维生素[J]. 食品研究与开发, 2012, 33(8): 138-140 |
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| 作者姓名： | 郭玉华 郁有祝 |
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| 作者单位： | 安阳工学院化学与环境工程系,河南安阳,455000 |
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| 摘 要： | 用高效液相色谱(HPLC)梯度洗脱法测定茶叶中的5种水溶性维生素成分和含量,结果表明,样品经XDB-C18柱分离,使用紫外检测器(波长266 nm),流动相为甲醇与0.03 mol/L KH2PO(4pH=6.1)混合,梯度洗脱,样品中5种水溶性维生素得到良好分离,该5种水溶性维生素标准曲线相关系数均达0.999 0以上,平均回收率为84.5%~102.7%,变异系数RSD<5.4%。测得普洱茶中VC的含量为14.37 mg/100g,VB1的含量为2.17 mg/100 g,VB6的含量为1.58 mg/100 g,VB2的含量为3.47 mg/100 g,叶酸含量为1.34 mg/100 g;信阳毛尖中VC的含量为11.69 mg/100 g,VB6的含量为1.76 mg/100 g,VB2的含量为8.12 mg/100 g,叶酸含量为9.66 mg/100 g,未检出VB1。
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| 关 键 词： | 高效液相色谱 水溶性维生素 茶叶 |
Determination of Five Water-Soluble Vitamins in Tea by High Performance Liquid Chromatography |
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| GUO Yu-hua , YU You-zhu. Determination of Five Water-Soluble Vitamins in Tea by High Performance Liquid Chromatography[J]. Food Research and Developent, 2012, 33(8): 138-140 |
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| Authors: | GUO Yu-hua YU You-zhu |
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| Affiliation: | (Department of Chemistry and Environmental Engineering,Anyang Institute of Technology,Anyang 455000,Henan,China) |
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| Abstract: | A HPLC gradient elution method with UV detector was established to determine five Water-Soluble Vitamins in the tea.The optimized conditions as follows: XDB-C18 column with methanol and 0.03 mol/L KH2PO4 buffer(pH=6.1) as mobile phase,and gradient elution,detector wavelength was 266 nm.The correlation coefficients were more than 0.999 0.The recoveries were 84.5 %-102.7 %,RSD<5.4 %.For vitamins C,B1,B2,B6 and Folic Acid,the contents were found to be 14.37 mg/100 g,2.17 mg/100 g,3.47 mg/100 g,1.58 mg/100 g and 1.34 mg/100 g in Puer tea;for Maojian tea,the contents of vitamins C,B2,B6 and Folic Acid were 11.69 mg/100 g,8.12 mg/100 g,1.76m g/100 g,9.66 mg/100 g,respectively.While vitamin B1 was not discovered in Maojian tea. |
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| Keywords: | high performance liquid chromatography water-soluble vitamins tea |
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