| HPLC法测定婴幼儿配方食品中的糠氨酸 |
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| 引用本文： | 于晓瑾,邢江涛,仇凯,钟其顶,李宇栋,熊正河,王艳萍.HPLC法测定婴幼儿配方食品中的糠氨酸[J].食品研究与开发,2012,33(2):158-161. |
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| 作者姓名： | 于晓瑾 邢江涛 仇凯 钟其顶 李宇栋 熊正河 王艳萍 |
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| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津,300457
2. 中国食品发酵工业研究院,北京100027;全国食品发酵标准化中心,北京100027 |
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| 摘 要： | 建立高效液相色谱法测定婴幼儿配方食品中糠氨酸的分析方法。样品经复溶后,经10.6 mol/L盐酸水解,用迪马铂金ODS色谱柱分离,采用紫外检测器在280 nm下进行检测,外标法定量。方法标准曲线线性相关系数r2=0.999 9,精密度RSD为2.23%,加标回收率为96.56%~106.24%,检出限(S/N=3)为0.38μg/mL,检测限(S/N=10)为1.25μg/mL。分析21个婴幼儿配方食品样品(含11个品牌),结果表明,糠氨酸含量水平在468 mg/100 g~1 467 mg/100 g(以蛋白质计)之间,部分样品的蛋白质质量损伤程度较大。
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| 关 键 词： | 高效液相色谱 婴幼儿配方食品 糠氨酸 |
Determination of Furosine in Infant Formula by HPLC |
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| YU Xiao-jin
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XING Jiang-tao
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QIU Kai
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ZHONG Qi-ding
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LI Yu-dong
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XIONG Zheng-he
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WANG Yan-ping.Determination of Furosine in Infant Formula by HPLC[J].Food Research and Developent,2012,33(2):158-161. |
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| Authors: | YU Xiao-jin XING Jiang-tao QIU Kai ZHONG Qi-ding LI Yu-dong XIONG Zheng-he WANG Yan-ping |
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| Affiliation: | 1(1.Institute of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;2.China National Research Institute of Food and Fermentation Industries,Beijing 100027,China;3.China National Food Fermentation Standardization Center,Beijing 100027,China) |
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| Abstract: | An analytical method of furosine in infant formula by high performance liquid chromatography was established.The samples were reconstituted with water,hydrolysed by 10.6 mol/L hydrochloric acid,separated by DIKMA Platisil ODS column,determined at 280 nm with UV detector,and calculated by external standard methods.This method has good linearity(r2=0.999 9),repeatability(RSD 2.23 %) and recovery(96.56 %-106.24 %).The detection limit(S/N=3) was 0.38 μg/mL and determination limit(S/N=10) was 1.25 μg/mL.The analysis results of the 21 infant formula samples(including 11 brands) showed that the content of furosine has a wide scope between in 468 mg/100 g-1 467 mg/100 g(by protein),the sample with higher fruosine content probably suffer serious heat damage and the nutrition quality may decreased. |
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| Keywords: | HPLC infant formula furosine |
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