富硒大米中硒形态分析

采用原子荧光光度法分析了天然富硒大米中硒的主要赋存形态、分布及含量.本实验中,硒的检测线性范围为4 μg/L～20μg/L,相关系数是r=0.999 3,总硒测定的相对标准偏差(RSD)为0.966％(n=6),总硒的加标回收率为103.12％.实验表明富硒大米中的总硒含量为206.708 μg/kg,达到60 μg/kg～300 μg/kg的富硒标准,而硒主要以对人体有益的有机硒形态存在,含量为168.875 μg/kg,占总硒的81.70％,而有机硒中最主要的赋存形态是蛋白硒,含量90.170 μg/kg,占53.40％,其次多糖硒占9.28％,RNA硒占1.86％,其它形态的有机硒占35.46％.

Analysis of Selenium Forms in Selenium-enriched Rice

Studied the combined forms,contribution and corresponding content of selenium in Selenium-enriched rice by using atomic fluorescence spectrometer.In the study,the linear calibrating curve for selenium was within the range of 4 μg/L-20 μg/L,whose correlative coefficient was 0.999 3,the RSD for total selenium was 0.966 %(n=6),the recovery rate of standard spiking for selenium was 103.12 %.The total selenium content was 206.708 μg/kg,which reached rich selenium standard of 60 μg/kg-300 μg/kg,and organic selenium was the main form storing in the rice,which content was 168.875 μg/kg,accounted for 81.70 % of total selenium,the selenium in protein was the main organic selenium form,whose content was 90.170 μg/kg,accounted for 53.40 %,secondly polysaccharide combined selenium accounted for 9.28 %,RNA combined selenium accounted for 1.86 %,and the other forms of organic selenium accounted for 35.46 %.