NaNO3对两种杜氏藻生长及色素积累的影响

研究NaNO3对两种杜氏藻Dunaliella salina和Dunaliella parva生长速度、色素积累的影响和NaNO3的吸收规律。结果表明,NaNO3浓度为1.5 mmol/L时,D.salina生长最快,最高密度76.4×104cell/mL;对照组为16.3×104cell/mL;低氮有利于D.salinaβ-胡萝卜素积累,β-胡萝卜素最大值105 mg/g出现在0.5 mmol/L NaNO3样中,对照组为45 mg/g;较高的NaNO3有利于叶绿素a的合成;藻液pH开始3 d~5 d急剧上升,后在波动中下降。对D.parva来说,NaNO3浓度为2.5 mmol/L时生长最快,细胞最高密度为295×104cell/mL,对照组为15×104cell/mL;在实验范围内,NaNO3浓度越高,β-胡萝卜素含量越高,β-胡萝卜素最大值为37.5 mg/g,叶绿素最大值为65 mg/g;建立了两种杜氏藻对NaNO3利用的动力学方程。

Effects of NaNO3 on Growth and Accumulation of Pigments in Two Kinds of Duanaliella Species

The effects of NaNO3 on the growth,pigment accumulation of two Dunaliella species and assimilated patterns of NaNO3 by two Dunaliella species were investigated.The optical growth of D.salina was observed in NaNO3 1.5 mmol/L and the highest cell density was detected to be 76.4×104 cell/mL,the control group only had the maximum cell density of 16.3×104 cell/mL.Low N content was favorable to accumulation of β-carotene of D.salina.The maximumβ-carotene of 105 mg/g occurred in 0.5 mmol/L NaNO3 media and the control group had the minimumβ-carotene of 45 mg/g.The pH values of D.salina media rose up rapidly in the first 3 d-5 d,then declined zigzagly.The optical growth of D.parva occurred in media with NaNO3 2.5 mmol/L and the maximum cell density was found to be 295×104 cell/mL and the control group had the minimum cell density of 15×104 cell/mL.The maximum β-carotene of 37.5 mg/g of D.parva was observed in media with 2.5 mmol/L NaNO3 and the maximum Chl a content of 65 mg/g was detected in the same media.The assimilated equations of NaNO3 by two Dunaliella species were set up.