| 团聚状纳米AuPd修饰的H2O2生物传感器研究 |
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| 引用本文： | 王孟,梁新义,冯苍松,张坤生.团聚状纳米AuPd修饰的H2O2生物传感器研究[J].食品研究与开发,2012,33(4):51-55. |
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| 作者姓名： | 王孟 梁新义 冯苍松 张坤生 |
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| 作者单位： | 1. 天津市食品生物技术重点实验室天津商业大学,天津,300134
2. 天津市食品生物技术重点实验室天津商业大学,天津300134;天津商业大学理学院应用化学系,天津300134 |
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| 基金项目： | 天津市自然科学基金(06YFJMJC12600);天津市自然科学基金重点项目(10JCZDJC23600);教育部留学回国人员科研启动基金资助项目(20071108) |
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| 摘 要： | 采用微胶束法室温条件下制备团聚状的AuPd合金纳米粒子,使用紫外可见光谱(UV-vis),透射电镜(TEM),X-射线粉末衍射(XRD)和X-射线能量色散谱(EDS)表征团聚结构AuPd合金纳米粒子的形貌、尺寸、结构和组成。用制备的AuPd纳米粒子修饰辣根过氧化物酶玻碳电极,制备无电子媒介的过氧化氢生物传感器HRP/AuPd/GCE。使用循环伏安法和计时电流法表征了HRP/AuPd/GCE对H2O2的检测性能。实验结果表明:该传感器对H2O2具有良好的检测性能和稳定性,在H2O2浓度为1×10-7mol/L～5×10-3mol/L范围内检测电流与H2O2浓度有线性关系,线性相关系数R2=0.995 01,检出限为7.6×10-7mol/L。
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| 关 键 词： | 团聚状AuPd合金纳米 H2O2检测 循环伏安法 辣根过氧化物酶 生物传感器 |
Study on Hydrogen Peroxide Biosensor Modified by Agglomerates of AuPd Nanoparticles |
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| WANG Meng
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LIANG Xin-yi
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FENG Cang-song
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ZHANG Kun-sheng.Study on Hydrogen Peroxide Biosensor Modified by Agglomerates of AuPd Nanoparticles[J].Food Research and Developent,2012,33(4):51-55. |
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| Authors: | WANG Meng LIANG Xin-yi FENG Cang-song ZHANG Kun-sheng |
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| Affiliation: | 1(1.Tianjin Key Laboratory of Food and Biotechnology,Tianjin University of Commerce,Tianjin 300134,China;2.Department of Applied Chemistry,College of Science,Tianjin University of Commerce,Tianjin 300134,China) |
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| Abstract: | The agglomerates of AuPd alloy nanoparticles were synthesized by microemulsion method at room temperature.The morphology,structure and composition were characterized by UV-vis,transmission electron microscopy(TEM),X-ray diffraction(XRD) and Energy dispersive x-ray spectroscopy(EDS).A electromediator-free hydrogen peroxide biosensor(HRP/AuPd/GCE) was prepared through modifying horseradish peroxidase electrode(HRP/GCE) by AuPd alloy nanoparticles.The results of cyclic voltammetry and i-t demonstrate that the HRP/AuPd/GCE exhibits direct electron transfer between HRP and GC electrode,and performs good sensi tivity and stability for detecting H2O2.The biosensor showed a linear response to H2O2in the range 1×10-7mol/L5×10-3mol/L with detection limit of 7.6×10-7mol/L,R2=0.995 01. |
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| Keywords: | AuPd aggregated nanoparticles detecting H2O2 cyclic voltammetry peroxide hydrogen biosensor |
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