纤维素酶-微波辅助提取软枣猕猴桃茎黄酮的工艺优化

采用纤维素酶-微波辅助法提取软枣猕猴桃茎中的黄酮类物质。通过单因素试验确定纤维素酶的添加量、酶解时间、作用温度、pH值的适宜水平,在此基础上,采用响应曲面法,通过方差分析建立数学模型,得到软枣猕猴桃茎黄酮提取的最佳工艺条件:纤维素酶添加量438U/g、酶解时间64min、作用温度49℃、pH4.7,在此条件下软枣猕猴桃茎黄酮提取率为2.89%。

Optimization of Cellulase Pretreatment for Microwave-Assisted Extraction of Flavonoids from Actinidia arguta Stem

Cellulase pretreatment before microwave-assisted extraction of flavonoids from Actinidia arguta stem was optimized by response surface methodology.One-factor-at-a-time experiments were performed to select appropriate levels of enzyme dose,hydrolysis time,temperature and pH.Further,a mathematical model was built and subjected to variance analysis.The optimal cellulase hydrolysis conditions were determined as follows: the optimal enzyme dose was 438 U/g for 64 min of hydrolysis at 49 ℃ and pH 4.7.Under these conditions,the extraction rate of flavonoids was up to 2.89%.