Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation |
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| Authors: | Ashman Ross Crimeen-Irwin Blessing Ludford-Menting Mandy Russell Sarah Gu Min |
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| Affiliation: | Centre for Micro-Photonics, School of Biophysical Sciences and Electronic Engineering, Swinburne University of Technology, Hawthorn, Australia. |
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| Abstract: | We show two-photon spectra and lifetimes acquired using conventional confocal microscopes equipped with an ultra-short pulsed laser and a time-gated intensified charge coupled device. We report on the two-photon spectra and lifetimes of Alexa350, enhanced green fluorescent protein (EGFP), EGFP-CD46, and Cy3 labelled antibodies. Cellular and extracellular EGFP two-photon spectra and lifetimes are compared. |
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| Keywords: | two‐photon microscopy lifetime imaging fluorescence resonance energy transfer |
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