| 荧光免疫传感器的初步研究 |
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| 引用本文： | 陆斌 韦枉. 荧光免疫传感器的初步研究[J]. 传感技术学报, 1989, 2(4) |
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| 作者姓名： | 陆斌 韦枉 |
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| 作者单位： | 东南大学(陆斌)，东南大学(韦钰) |
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| 摘 要： | 本文较详细地分析了荧光免疫传感器表面膜的作用机理,给出了传感器检测灵敏度与动态范围理论值,建立了传感器检测系统,讨论了表面免疫反应的时间特性及非特异性蛋白吸附作用、吸附的消除及对传感器性能的影响,并比较了两种解离剂对表面膜使用重复性的影响。最后给出了传感器检测动态范围的实验数据,结果与理论值一致。
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| 关 键 词： | 抗体 抗原 免疫传感器 荧光 竞争反应 |
The Preliminary Study of Fluoro-Immnnosensor |
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| Lu Bin Wei Yu. The Preliminary Study of Fluoro-Immnnosensor[J]. Journal of Transduction Technology, 1989, 2(4) |
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| Authors: | Lu Bin Wei Yu |
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| Affiliation: | Southeast Univ. |
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| Abstract: | The characteristics and system of a new kind of biosensor, fluoto-immuno sensor have been studied. In this paper reaction mechanisms of surface sensitive film are theoretically analyzed and the theoretical value of detective ensiivity and dynamic range of the sensor are given. The detective system is built and the sensing function is overall examined. Several properties of the sensor are discussed including binding and eluting of suiface antigen with antibody adsorption of non-specific protein, the influence of the adsorpton to the functions of the sensor and the reuse of sensor. Finally the testing results of the detective dynamic range is givea. The results correspond with the theoretical values. |
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| Keywords: | antibody antigen immunosensor fluorescence competition reaction |
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