菊花多糖对2型糖尿病大鼠的降血糖作用

目的:研究菊花多糖对2型糖尿病(T2DM)大鼠的降血糖作用。方法:利用水提醇沉法对菊花多糖进行提取,高脂高糖饲料饲养大鼠6周后,一次性腹腔注射STZ(30 mg/kg·BW),建立T2DM大鼠模型,试验过程中记录大鼠体重、摄食量、饮水量,每2周测定大鼠空腹血糖值(FBG),试验最后1周进行糖耐量(OGTT)试验,采用Elisa法测定大鼠胰岛素(INS)水平,并计算胰岛素抵抗指数(HOMA-IR)。测定大鼠血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平,以及超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)等水平,采用蛋白质免疫印迹法(Western Blot)检测Nrf2、Keap1、HO-1的表达水平。结果:经菊花多糖治疗后,能够缓解T2DM大鼠体重的下降,减少其摄食量和饮水量,提高T2DM大鼠糖耐量,降低大鼠INS及HOMA-IR。能够显著降低T2DM大鼠TC、TG、LDL-C水平(P<0.05),并显著提高HDL-C水平(P<0.05)。T2DM大鼠SOD、GSH、CAT水平有显著提高(P<0.05),MDA水平显著下降(P<0.05)。经菊花多糖治疗后的T2DM大鼠的Nrf2、Keap1、HO-1蛋白水平均趋于正常组的蛋白水平。结论:菊花多糖能够有效改善T2DM大鼠“三多一少”的症状,降低FBG水平,提高OGTT,改善INS水平及HOMA-IR,改善T2DM大鼠异常脂代谢和氧化应激水平,并通过调控Nrf2/Keap1/HO-1通路相关蛋白起到治疗T2DM的作用。

Study on the effect of chrysanthemum polysaccharide on hypoglycemic in type 2 diabetic rats

Objective:This study aimed to investigate the hypoglycemic effect of chrysanthemum polysaccharide on type 2 diabetes(T2DM)rats.Methods:The chrysanthemum polysaccharides were extracted by water extraction and alcohol precipitation.After feeding rats with high-fat and high-sugar feed for 6 weeks,STZ(30 mg/kg·BW)was injected intraperitoneally to establish a T2DM rat model,and records were recorded during the experiment.Rat body weight,food intake,water consumption,fasting blood glucose(FBG)was measured every two weeks,and glucose tolerance(OGTT)test was performed in the last week of the test;the insulin(INS)level was measured by Elisa method,and insulin resistance was calculated Index(HOMA-IR).Determination of total cholesterol(TC),triglycerides(TG),high-density lipoprotein(HDL-C),low-density lipoprotein(LDL-C)levels,as well as the levels of superoxide dismutase(SOD),cystathione(GSH),catalase(CAT),and malondialdehyde(MDA)were detected by Western Blot to detect the expression levels of Nrf2,Keap1,and HO-1.Results:Treatment with chrysanthemum polysaccharides could alleviate the weight loss of T2DM rats,reduce their food intake and water consumption,improve the glucose tolerance of T2DM rats,and reduce INS and HOMA-IR in rats.It could significantly reduce the levels of TC,TG,LDL-C in T2DM rats(P<0.05),and significantly increase the level of HDL-C(P<0.05).The levels of SOD,GSH and CAT in T2DM rats increased significantly(P<0.05),and the level of MDA decreased significantly(P<0.05).The Nrf2,Keap1,and HO-1 protein levels of T2DM rats treated with chrysanthemum polysaccharides tended to normal protein levels.Conclusion:Chrysanthemum polysaccharide can effectively improve the symptoms of"three more and one less"in T2DM rats,reduce FBG levels,increase OGTT,improve INS levels and HOMA-IR,improve abnormal lipid metabolism and oxidative stress levels in T2DM rats,and through regulation Nrf2/Keap1/HO-1 pathway related proteins play a role in the treatment of T2DM.