乙醇抑制法提取低钾Kappa-卡拉胶

目的:以乙醇抑制卡拉胶溶解替代钾离子凝胶法提取卡拉胶。方法:对卡拉胶提取过程中的碱处理、清洗残碱及过滤后沉淀卡拉胶等工序的乙醇体积分数进行优化,并探索卡拉胶中钾离子的控制方法。结果:碱处理及清洗残碱过程中抑制卡拉胶溶解的最佳乙醇体积分数为60%,卡拉胶沉淀过程中60%乙醇溶液与胶液体积比(V_60%乙醇∶V_胶液)为5∶1;该工艺下卡拉胶得率为(28.76±1.38)%,显著高于传统工艺的(22.9%);钾离子浓度为0.63%,显著低于传统工艺提取的(16.26%);黏度、硬度、弹性、咀嚼性和回复性与传统工艺提取的无显著差异。结论:乙醇抑制法提取的Kappa-卡拉胶K⁺浓度和凝胶强度显著低于传统工艺提取的。

Study on the extraction of low-potassium Kappa-Carrageenan by ethanol inhibition

Objective:In order to control the potassium ion content in Carrageenan,this project used alcohol inhibition method instead of potassium ion solution to extract Carrageenan.Methods:The volume fraction of ethanol in the processes of alkali treatment,alkali residual cleaning,and precipitation of carrageenan after filtration during the extraction were optimized for controlling K+concentration.Results:The concentration of ethanol to inhibit the dissolution of Carrageenan was 60% in the process of alkali treatment and cleaning residual alkali,and the ratio of 60% ethanol solution to glue solution was 5∶1 in the process of Carrageenan precipitation.By using this process,the yield of Carrageenan was(28.76±1.38)%,and the Carrageenan of potassium ion was 0.63%,which was significantly lower than that of potassium ion extracted from traditional Carrageenan(16.26%).In addition,the gel strength was significantly lower than that of Carrageenan extracted by traditional methods.And there was no significant difference in viscosity,hardness,elasticity,chewiness,and recovery between the two methods.Conclusion:K⁺concentration and gel strength of Kappa-carrageenan extracted by ethanol inhibition were significantly lower than traditional technology.