A new colorimetric assay for antioxidant capacity and photostability |
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| Authors: | Elisabet Fernández‐García Hayat Heluani‐Gahete Ralf E. Wellinger |
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| Affiliation: | Andalusian Centre of Molecular Biology and Regenerative Medicine (CABIMER), University of Seville, 41092 Seville, Spain |
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| Abstract: | The antioxidant capacity of cysteine, glutathione, N‐acetylcysteine, and ascorbic acid to reduce Methylene Blue to colourless Leucomethylene Blue was analysed. Interestingly, transient exposure of Leucomethylene Blue to sunlight resulted in a reversible oxidation reaction and thus, provided a fast, easy, and simple quantitative assay to determine antioxidant capacity and photostability of biologically relevant antioxidants. |
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