油茶蒲醇提纯化物的组分鉴别及对油脂的抗氧化作用

利用S-8大孔树脂对油茶蒲醇提物进行纯化，再采用HPLC对油茶蒲醇提纯化物（POSE）中的功能组分进行鉴定，然后研究油茶蒲醇提纯化物对3种不同类型植物油的抗氧化作用，并与TBHQ进行对照。结果表明：油茶蒲醇提物经过S-8大孔树脂纯化后，总黄酮含量由22.41%提高到66.86%，总黄酮回收率达89.14%,总黄酮得率达4.68%；油茶蒲醇提纯化物中初步鉴定出5种功能成分，其中没食子酸含量为18.83 mg/g，儿茶素含量为26.12 mg/g，表儿茶素含量为27.64 mg/g，芦丁含量为16.13 mg/g，槲皮素含量为0.52 mg/g；油茶蒲醇提纯化物能有效抑制油脂的氧化酸败，与对油茶籽油和菜籽油的抗氧化作用相比，油茶蒲醇提纯化物对富含高不饱和脂肪酸的核桃油抗氧化作用较好。因此，油茶蒲醇提纯化物具有作为高不饱和脂肪酸型油脂的抗氧化剂开发的潜力。

Component identification and antioxidant effects on edible oils of purified ethanol extract of Camellia oleifera fruit hull

The S-8 macroporous resin was used to purify the ethanol extract of Camellia oleifera fruit hull. The functional components of purified Camellia oleifera fruit hull extract(POSE) were identified by HPLC. Then the antioxidant effect of POSE on different types of vegetable oils was studied and compared with TBHQ. The results showed that the total flavonoid content of Camellia oleifera fruit hull extract increased from 22.41% to 66.86% after the purification by S-8 macroporous resin, and the recovery rate and the yield of total flavonoids reached 89.14% and 4.68%,respectively. POSE had five functional components including gallic acid 18.83 mg/g, catechin 26.12 mg/g, epicatechin 27.64 mg/g, rutin 16.13 mg/g and quercetin 0.52 mg/g. POSE could effectively inhibit the oxidative rancidity of oils. Compared with the antioxidant effects on oil-tea camellia seed oil and rapeseed oil, POSE had better antioxidant effect on walnut oil rich in polyunsaturated fatty acids. Therefore, as a natural plant product, POSE has the potential to be developed as an antioxidant of oils with high unsaturated fatty acids.