| ZIF-8原位自封装固定化脂肪酶的研究 |
| |
| 引用本文： | 梁鑫,张成楠,周威,李秀婷,万澄莹. ZIF-8原位自封装固定化脂肪酶的研究[J]. 食品研究与开发, 2021, 42(5): 1-8 |
| |
| 作者姓名： | 梁鑫 张成楠 周威 李秀婷 万澄莹 |
| |
| 作者单位： | 北京食品营养与人类健康高精尖创新中心;北京工商大学食品与健康学院;北京工商大学理学院;北京市食品添加剂工程技术研究中心 |
| |
| 基金项目： | 国家自然科学基金(31830069、3200161409);北京市教育委员会科技计划一般项目(KM202010011007);北京工商大学食品与健康学院人才培养质量建设-一流专业建设-食品科学与工程(19002020119)。 |
| |
| 摘 要： | 该研究使用沸石-咪唑框架-8(zeolitic imidazolate framework-8,ZIF-8)多孔结构材料原位自封装皱褶假丝酵母源脂肪酶(Candida rugosa lipase,CRL)制备固定化脂肪酶(CRL@ZIF-8),利用红外光谱(Fourier transform infrared spec...
|
| 关 键 词： | 脂肪酶 沸石-咪唑框架-8(ZIF-8) 原位封装法 2-甲基咪唑 醋酸锌 |
| 收稿时间： | 2020-04-05 |
Study on Immobilization of Lipase by In-situ Encapsulation in ZIF-8 |
| |
| LIANG Xin,ZHANG Cheng-nan,ZHOU Wei,LI Xiu-ting,WAN Cheng-ying. Study on Immobilization of Lipase by In-situ Encapsulation in ZIF-8[J]. Food Research and Developent, 2021, 42(5): 1-8 |
| |
| Authors: | LIANG Xin ZHANG Cheng-nan ZHOU Wei LI Xiu-ting WAN Cheng-ying |
| |
| Affiliation: | (Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University(BTBU),Beijing 100048,China;School of Food and Health,Beijing Technology and Business University(BTBU),Beijing 100048,China;School of Science,Beijing Technology and Business University(BTBU),Beijing 100048,China;Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University(BTBU),Beijing 100048,China) |
| |
| Abstract: | In the present study,Candida rugosa lipase(CRL)was immobilized into ZIF-8 by in-situ encapsulation.The effects of metal ion source,reaction time,concentrations of metal ion and organic ligands on the catalytic activity and morphology of immobilized enzyme CRL@ZIF-8 were studied by using Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction(XRD)and scanning electron microscopy(SEM).The conditions of immobilized lipase were obtained:the concentration of 2-methylimidazole was 1.0 mol/L,the concentration of zinc acetate was 0.5 mol/L,and the reaction time was 30 min.The activity of the synthesized CRL@ZIF-8 enzyme reached 9.8 U/g.The temperature stability of the immobilized lipase CRL@ZIF-8 was significantly improved compared with free CRL. |
| |
| Keywords: | lipase zeolitic imidazolate framework-8(ZIF-8) in-situ encapsulation 2-methylimidazole zinc acetate |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《食品研究与开发》浏览原始摘要信息 |
|
点击此处可从《食品研究与开发》下载全文 |
|
