氧化硫硫杆菌的培养特性及低品位磷矿浸出

从安徽某煤矿的酸性矿坑水中分离出能有效浸出低品位磷矿的氧化硫硫杆菌菌株,对其形态特征和培养特性进行了初步的研究,并通过研究不同固体培养基成分对氧化硫硫杆菌分离纯化的影响,确定了适宜的分离条件：采用双层平板,即上层为涂布氧化硫硫杆菌的Starkey固体培养基平板,下层为涂布异养菌（红酵母菌）的琼脂平板,可显著提高菌落密度并缩短检出时间。考查了培养基、表面活性剂等因素对所选育的氧化硫硫杆菌的浸磷效果的影响,通过初步试验浸磷率可达到48.41%。

Cultivation Characteristics of Acidithiobacillus Thiooxidans and Bioleaching of Low-grade Phosphate Ore with It

A strain of Acidithiobacillus thiooxidans(At.t) that can be used effectively in the leaching of low-grade phosphate ore was isolated from acid mine drainage from a coal mine in Anhui.Its characteristics of morphology and cultivation were studied.Through the investigation of the effect of different solid culture medium components on the isolation and purification of Acidthiobacillus thiooxidans,the optimum isolation conditions were determined by using a double-layer plate with chemoautotrophic bacterium At.t spread onto the upper Starkey solid culture medium layer,and heterotrophic microbe Rhodotorula sp.spread onto the bottom agar layer,resulting in significantly increased density and reduced forming time of the bacterial colony.The influence of factors including culture media and the addition of surfactant on the leaching of phosphate ore with the cultivated At.t was studied.Through preliminary experiments,up to 48.41% of phosphate in the ore was leached out by using the isolated At.t strain.