Emulsion Stability as Affected by Competitive Adsorption Between an Oil-Soluble Emulsifier and Milk Proteins at the Interface |
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| Authors: | MICHEL CORNEC PETER J. WILDE PAUL A. GUNNING ALAN R. MACKIE FIONA A. HUSBAND MARY L. PARKER DAVID C. CLARK |
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| Affiliation: | The authors are affiliated with the Food Biophysics Department, Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney Lane, Norwich NR4 7UA, England. |
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| Abstract: | The effect of an oil-soluble emulsifier upon adsorption properties of β-casein and β-lactoglobulin at the oil-water interface was studied. Addition of emulsifier improved the emulsification properties, but lowered emulsion stability. Higher concentrations decreased emulsification capacity, due to an increase in oil phase viscosity, and interfered with several protein assays, except the o-phthaldialdehyde (OPA) assay. Depletion measurements revealed that the emulsifier displaced β-casein more readily than β-lactoglobulin from the surface of droplets, which correlated with emulsification and stability measurements. Displacement of proteins was not complete due to emulsifier-induced formation of a multiple w-o-w emulsion, which accounted for the missing protein in the continuous phase. |
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| Keywords: | milk proteins surfactant competitive adsorption emulsion stability |
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