Methods for characterizing plant fibers |
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Authors: | Cruthers Natasha Carr Debra Niven Brian Girvan Elizabeth Laing Raechel |
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Affiliation: | Clothing and Textile Sciences, University of Otago, Dunedin, New Zealand. |
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Abstract: | The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual plant, a fourth leaf from a north-facing fan, age of plant, and cultivars (two). Experimental variables were microscopy techniques and measurement axis. Measurements of width and length of harakeke ultimate fibers depended on the microscopic preparation/technique used as well as the cultivar examined. The best methods were (i) transverse sections of leaf specimens 4 microm thick, embedded in Paraplast and observed using light microscopy, and (ii) nonfixed ultimate fibers observed using scanning electron microscopy. |
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Keywords: | harakeke New Zealand flax field emission scanning electron microscopy light microscopy |
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