Production of cyclic tetrasaccharide from starch using a novel enzyme system from Bacillus globisporus C11 |
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Authors: | Aga Hajime Higashiyama Takanobu Watanabe Hikaru Sonoda Tomohiko Nishimoto Tomoyuki Kubota Michio Fukuda Shigeharu Kurimoto Masashi Tsujisaka Yoshio |
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Affiliation: | Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami-machi, Okayama 700-0834, Japan. amaseken@hayashibara.co.jp |
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Abstract: | Production of cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->] (CTS, cyclic tetrasaccharide) from starch was attempted using 1,6-alpha-glucosyltransferase (6GT) and 1,3-alpha-isomaltosyltransferase (IMT) from Bacillus globisporus C11. The optimal conditions for production from partially hydrolyzed starch were as follows: substrate concentration, 3%; pH 6-7; temperature, 30 degrees C; 6GT, 1 unit/g-dry solid (DS); IMT, 10 units/g-DS. The production of CTS was demonstrated and 544 g of CTS hydrate crystal powders were obtained from 3500 g of partially hydrolyzed starch. Two major by-products were also isolated from the reaction mixture and identified as the branched derivatives of CTSs, 4-O-alpha-D-glucopyranosyl-CTS and 3-O-alpha-isomaltosyl-CTS. |
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