大孔吸附树脂纯化石榴皮多酚

从D3520、D4020、AB-8、D140、D141、D160、DM-301、DA-201、SAD-7和D101大孔吸附树脂中筛选出D141树脂,研究了其对石榴皮多酚的静态与动态吸附和解吸性能。结果表明,D141树脂对石榴皮多酚的饱和吸附量为19.86 mg/g(干树脂),吸附等温线符合Langmuir方程,饱和吸附时间为5 h,适宜解吸剂为体积分数70%的乙醇溶液;以质量浓度9 mg/mL的石榴皮提取液上柱,流速为1.8~2.0 BV/h时,树脂的多酚穿透吸附容量为39.42 mg/g(干树脂),2.5 BV体积分数70%的乙醇溶液可将吸附于柱上的石榴皮多酚完全洗脱。以该条件纯化石榴皮多酚提取物时,纯化样的收率为15.4 g/100 g(石榴皮),多酚质量分数从34%提高到76.34%。

Purification of Polyphenols from Pomegranate Peel by Macroporous Adsorbent Resin

From 10 kinds of macroporous adsorbent resins including D3520,D4020,AB-8,D140,D141,D160,DM-301,DA-201,SAD-7 and D101,D141 resin was selected as a suitable material to purify the polyphenols from pomegranate peel.The static saturated adsorption capacity of D141 resin was 19.86 mg/g dry resin while the time for it to achieve saturation adsorption was 5 h.Besides,the adsorption isotherm data of D141 resin could well fit the Langmuir equation.70%(volume fraction) ethanol was a suitable solvent to elute the polyphenols adsorbed on the resin.The breakthrough adsorption capacity of the resin was 39.42 mg/g dry resin at a feeding concentration of 9 mg/mL and a flow rate of 1.8-2.0 BV/h.2.5 BV of 70%(volume fraction) ethanol could completely elute the polyphenols adsorbed on the column.When pomegranate peel extract was purified under this condition,the yield of purified sample was 15.4 g/100 g dry peel with the polyphenol mass fraction increasing from 34% to 76.34%.