| 高效液相色谱测定水解大豆中异黄酮方法研究 |
| |
| 引用本文： | 张晓波,吴岩,林红. 高效液相色谱测定水解大豆中异黄酮方法研究[J]. 粮食与油脂, 2006, 0(4): 19-21 |
| |
| 作者姓名： | 张晓波 吴岩 林红 |
| |
| 作者单位： | 黑龙江省农业科学院,哈尔滨,150086 |
| |
| 摘 要： | 该研究建立大豆提取物中大豆异黄酮高效液相色谱测定方法,通过正交实验确立糖苷型大豆异黄酮转化为游离型大豆异黄酮最佳酸水解工艺条件:盐酸浓度为2．0 mol/L,水解温度为80℃, 水解时间为1．5 h;采用ZorbaX 80A Extend-C18 4．6×150 mm 4 μm色谱柱,MeOH-1．8％冰乙酸水溶液(35:65,V／V)为流动相,MeOH 35％-50％梯度洗脱,流速1．0 ml/min,检测波长为260nm等色谱条件下测定甙元含量,并通过换算因子计算大豆异黄酮含量。
|
| 关 键 词： | 大豆异黄酮 糖苷型大豆异黄酮 酸水解 高效液相色谱法 |
| 文章编号： | 1008-9578(2006)04-0019-03 |
| 修稿时间： | 2006-03-05 |
Study on Method of Hydrolyze Isoflavone in Soybean by HPLC |
| |
| ZHANG Xiao-bo,WU Yan,LIN Hong. Study on Method of Hydrolyze Isoflavone in Soybean by HPLC[J]. Cereals & Oils, 2006, 0(4): 19-21 |
| |
| Authors: | ZHANG Xiao-bo WU Yan LIN Hong |
| |
| Abstract: | A method on distilling isoflavone in soybean by HPLC is found in this article. By means of orthogonal test, the condition for hydrolyzing soybean isoflavones glycoside into isoflavones aglycon was optimizedas follows:2.0 mol/L hydrochloric acid, temperature 80 ℃ , time of hydrolyze 1.5 hour, chromatogram pillar is Zorbax 80A Extend-C18 4.6×150 mm 4 μm,flow liquid MeOH-1.8%(acetic acid : water is 35 : 65, v/v), MeOH 35% -50% grads cleanout flow-rate of 1.0 ml/min, wavelengh 260 nm, account soflavone in soybean by conversion gene. |
| |
| Keywords: | soybean isoflavone soybean isoflavone glycoside acid hydrolyzation HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
