利用SMART RACE克隆STGA3 cDNA的5′末端

阐述了SMART RACE的原理,克隆了草莓贝壳杉烯氧化酶基因(STGA3)5′端未知序列。所得到的STGA3 cDNA的5′末端共有1 474 bp,其中非编码区共34 bp,编码区1 440 bp,编码氨基酸480个,此序列在NCBI的注册号为:AY462247。它与已克隆到的同源基因拟南芥GA3和南瓜CYPT01A1之间的同源性分别为56.9%和59.2%。

Cloning STGA3 cDNA 5′ End by SMART RACE Technique

The principle of SMART RACE was described.The 5′sequence of strawberry ent-kaurene oxidase(STGA3)was cloned by using this technique.The cloned STGA3 5′end cDNA was 1 474 bp long,including 34 bp non-encoding region and 1 440 bp encoding region which encoded 480 amino acids.The deduced protein was 56.9% and 59.2% homologus to the GA3 in Arabodpsis and CYPT 01A1 in Cucurbita maxima respectively.