微波预处理水酶法提取茶叶籽油工艺优化

研究微波处理茶叶籽仁，水酶法提取茶叶籽油的工艺条件。茶叶籽仁粉碎60目加6倍质量的水，经过800W微波处理10min，加入纤维素酶1.5%、果胶酶2.0%、蛋白酶0.25%，采用pH4.5、酶解温度45℃、酶解6h，离心萃取茶叶籽油。结果表明，微波预处理茶叶籽能够促进水酶法提取茶叶籽油，出油率达27.9%。

Optimization of Microwave Pretreatment Followed by Aqueous Enzymatic Extraction of Tea Seed Oil

The present paper describes an investigation into the extraction of tea seed oil by microwave pretreatment followed by aqueous enzymatic extraction.Tea seeds were pulverized to 60 mesh and added to a 6-fold volume of deionized water before microwave treatment for 10 min at a power level of 800 W.Thereafter cellulase,pectinase and acid protease were added at respective doses of 1.5%,2.0% and 0.25% for hydrolysis at pH 4.5 and 45 ℃ for 6 h.Finally centrifugation and extraction were sequentially conducted to obtain tea seed oil.Microwave pretreatment could promote the aqueous enzymatic extraction of tea seed oil,resulting in an extraction yield of 27.9%.